Effects of cryogenic treatment,pre-annealing cycling treatment and cryogenic-pre-annealing cycling treatment on the microstructure and crystalline phase precipitation in Fe-based amorphous alloy were investigated by DSC（differential scanning calorimetry）,TEM（transmission electron microscope）and SEM（scanning electron microscope）.The results reveal that crystallization parameters,such as Tgand Tx,of amorphous alloy are changed by cycling treatment,and the enthalpy relaxation peak appears in the DSC testing curves.The samples pre-treated still remain to be amorphous with the precipitation of nano-crystalline phase.The pre-treatment can result in the mergence and size increase of clusters,leading to the formation of grape-like structure of cluster in amorphous alloy.Besides,different types and scales of crystalline phase precipitation are observed in the amorphous alloy pre-treated.It is believed that the pre-treatment method leads to the formation of new cluster and the mergence and growth of clusters by element diffusion,changing the crystallization thermo-dynamics and crystallization kinetics of amorphous alloy.